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Targeting SIRT1 rescues age- and obesity-induced microvascular dysfunction in ex vivo human vessels

L-Glutathione Oxidized (Glutathione Disulfide, GSSG) Special type of Glutathione, odorless and easily dissolved in water

HAoEC were cultured in a complete EGM-2MV medium (Lonza, Basel, Switzerland) that had the following supplementations: 10 mM L-glutamine (Gibco Life Technologies), 1 g/mL hydrocortisone (Sigma Aldrich), 10 mg/mL epidermal growth factor (EGF, Sigma Aldrich), 10% fetal bovine serum (FBS, Gibco Life Technologies), and 1% of antibiotics (streptomycin, penicillin, and fungison, Gibco Life Technologies)

Cu + was used to react with H 2 O 2 to exert CDT