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Glucose and insulin signaling: Evaluation within metabolic research frameworks

Proteins were extracted by using RIPA buffer supplemented with phosphatase and protease inhibitors, quantified by using Bradford method (23236, ThermoFisher)

When methylene blue is taken at a dose of 2 mg/kg, it is considered safe, but if the levels exceed 7 mg/kg, then it can lead to various adverse effects

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