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Column: C18 150 4.6 mm 5 m Mobile phase: Water (0.1% TFA) / Acetonitrile (0.1% TFA) Gradient: 3565% ACN Flow rate: 1.0 mL/min Detection: UV 214 nm Injection volume: 10 L Run time: 15 minutes Application: Quantification and purity analysis of semaglutide in pharmaceutical formulations

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