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The sequencing reads were deduplicated using umi tools (v1.1.6), mapped to the reference plasmid using BWA-MEM, and further cleaned using fgbio (v2.4.0) to remove the soft-clip sequences
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Katsuoka, F
Tanycytic VEGF-A boosts bloodhypothalamus barrier plasticity and access of metabolic signals to the arcuate nucleus in response to fasting
