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Description
Identified in 1973 as a wound-healing modulator, GHK-Cu has since emerged as a key molecule in tissue regeneration, stem-cell activation, anti-inflammatory signaling, and epigenetic gene modulation....
Results A counter-selection system was engineered in Saccharomyces cerevisiae based on the known feedback inhibition of gamma-glutamylcysteine synthetase (GSH1) gene expression, which is rate limiting for GSH synthesis: the GSH1 ORF and the counter-selectable marker GAP1 were expressed under control of the TEF1 and GSH-regulated GSH1 promoters, respectively
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