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The induction of EMT was monitored by the upregulation of N-cadherin which was accompanied by a strong and permanent decrease of LAP levels and a slight and transient decrease in LIP levels resulting in a strong increase of the LIP/LAP ratio at the end of the treatment period (Fig

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Nuclei were isolated from frozen, ground plant tissue using ice-cold Honda buffer (0.44 M sucrose, 1.25% (w/v) Ficoll, 2.5% (w/v) dextran T40, 20 mM HEPES-KOH pH 7.4, 10 mM MgCl 2 , 0.5% (w/v) Triton X-100, 1 mM DTT, 1 protease inhibitor (Roche) and 0.4 U l 1 Ribolock RNase inhibitor (Thermo Fisher Scientific)) and collected via centrifugation at 2,000 g for 5 min at 4 C