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Description
Low nanomolar concentrations of PregS induce a delayed onset increase of the neuronal response to NMDA and trafficking of NMDAR to the cell surface through an intracellular Ca 2+ ([Ca 2+ ]i)-dependent and non-canonical mechanism involving G-proteins (47) (Figure 2)
RNA isolation and qRT-PCR Total RNA from the tissues and cells was extracted using the TRIzol method (Takara, Japan)

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