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129 Therefore, we suggest that the disruption of cholesterol metabolism causes lysosomal dysfunction, which leads to autophagy dysfunction
A prepacked Ni-NTA column was equilibrated with dialysis buffer and the flowthrough was collected and concentrated to 2 mL using a 10 kDa MWCO concentrator (Amicon Ultra 15, Millipore)

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But, as is typical with many of the micronutrient guidelines in pregnancy, newer research has found that a much higher amount of vitamin B12 is ideal
