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10.1016/j.tox.2017.05.019 Toxicology 36 FuZ
Sometimes this issue is fixed simply by optimizing buffers, for instance by increasing salt or detergent concentration, so that the interacting protein no longer binds to the GST only control, but still binds to your GST-fusion protein of interest

A significant improvement in the periorbital appearance was observed in 82.1% of participants presenting vascular dark circles, suggesting the potential benefit of carboxytherapy for bluish discoloration

doi: 10.1634/theoncologist.2019-0057
