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Metabolic surgery offers rapid metabolic improvements

Lacey carbon EM grids were glow-discharged (30 s, 25 mA) in the Pelco EasiGlow machine to prepare samples for cryo-EM research

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This is attributed to the flexible compensatory mechanism adopted by PDAC to overcome metabolic perturbations, which includes increasing expression of alternative enzyme isoforms and dependencies 41,42 on alternative metabolic pathways, such as glutamine metabolism 43,44,45