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Its structure was derived from growth hormone releasing peptide-1 (GHRP-1) by removing the central Ala-Trp dipeptide and introducing aminoisobutyric acid (Aib) at the N-terminus for metabolic stability

Culture media consisted of 10 g/L glucose, 1 g/L yeast extract, 5 g/L (NH 4 ) 2 SO 4 , 2 g/L KH 2 PO 4 , 0.5 g/L MgSO 4 7H 2 O, 0.1 g/L CaCl 2 2H 2 O, 0.02 g/L FeSO 4 7H 2 O, 0.01 g/L ZnSO 4 7H 2 O, 0.005 g/L CuSO 4 5H 2 O, 0.001 g/L MnCl 2 4H 2 O, 2.5 mL of 70% H 2 SO 4 and 0.5% (v/v) Antifoam 204 (Sigma Aldrich, St