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Immunofluorescence Fibroblast cultures at passage 3 were cultured in eight-well chamber slides (Gibco, Burlington, ON, Canada) in growth medium (DMEM, 10% fetal bovine serum (FBS), penicillin, and streptavidin from Invitrogen, Burlington, ON, Canada)

Daratumumab and eculizumab combined therapy reduced dn DSAs, improved heart and kidney graft function, and resulted in undetectable circulating PCs