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(5) Gently invert the culture plate and incubate it in a CO 2 incubator at 37 , 5% CO 2 , and 80% relative humidity for 30 minutes to allow the matrix gel to polymerize
Previous studies investigating TMAO responses after an OCC did not measure TMAO between 8 and 24 h and assumed T max occurred at 24 h (22, 33)

Available here Gill MJ, Arlette J, Buchan K

Most of the work, however, was done under project No