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Anti-cancers Methotrexate It was revealed that mice received methotrexate resulting in interstitial space dilatation, edema, vasculitis, abnormalities of Leydig cells, severe disruption of the seminiferous epithelium, and reduced diameter of the seminiferous tubules
The ultrasound stimulates microbubbles to oscillate violently and burst, producing a temporary, local pressure change that can temporarily open the tight junctions of the BBB and increase its permeability

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Despite this well-established safety, some negative side effects have been reported