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Description
A single brief excursion during travel or a power outage is probably acceptable
CHIR99021 was removed after 72 h and DE cells were cultured in RPMI/B27 + Insulin for 24 h (Siller et al., 2015
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The 3D culture medium was xeno-free CMRL 1066-media with the CIT (Clinical Islet Transplant Consortium) modifications with added IGF-1 heparin, and human serum albumin
