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Histology and immunostaining Mice were cardiac-perfused with 0.1 M phosphate-buffered saline (PBS, pH 7.4) followed by 4% paraformaldehyde (PFA) in phosphate buffer

Importantly, we established that ultrafiltered flavan-3,3,4-triol (4 ) was converted to cyanidin ( 8 ) by additional well-characterized arGSTs from maize ( Zm BZ2), arabidopsis ( A tTT19) and grapevine ( Vv GST4), and from the structure-elucidated phi-class orthologue from poplar ( Pt GSTF8), while application of GSTs unrelated to anthocyanin biosynthesis ( Vv GST2, Vv GST5, At GSTF2) did not result in cyanidin ( 8 ) formation (Fig

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