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Unlike traditional growth factors, TB-4 is small, freely diffusible, and not limited to specific receptor targets

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Mitochondrial oxygen consumption assay 100 g of isolated intact mitochondria in cytosolic extracts of reticulocyte cells containing 0.6 M sorbitol, 20 mM HEPES/KOH (pH 7.4) and 1 mM MgCl2 was used for oxygen consumption assay