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Vitamin B12 Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of Vitamin B12 in biofluid samples, such as serum, plasma, tissue homogenates and other biological fluids. This is a competitive inhibition Sandwich ELISA in strip well format with reagents for up to 96 tests . VB12 present in samples reacts with an anti-VB12 monoclonal capture antibody or antigen coated and dried on a microtiter plate. Following this, VB12 conjugated to biotin competes with the antigen in the sample to bind the capture antibody or antigen. Unbound conjugate and sample antigen is washed away and bound conjugate is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is inverse proportional to the concentration of active VB12 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of VB12. Detection Target: Vitamin B12 Reactivity: Multi-species Range: 4.68-300ng/mL Sensitivity: 3.8ng/mL Storage Conditions: Store all components at 4C This Multi-species VB12 activity assay is for the quantitative determination of Vitamin B12 in samples including serum, plasma, tissue homogenates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results

Search strategy We conducted searches in Medline, Extended Medline (1950 to 22 July 2020) using the following search words (Vitamin B12 or Cobalamin or Methylcobalamin or Cyanocobalamin) and (Autism, Autistic spectrum disorder or attention deficit hyperactivity disorder or ASD or ADHD)

CoQ10 Sustains cellular energy, Heart health, Antioxidant defense $40 Read Benefits Boosts energy production in cells Supports heart health and cardiovascular function Acts as a powerful antioxidant, protecting cells from damage Enhances athletic performance and physical endurance Promotes healthy skin and reduces signs of aging Improves mitochondrial function Supports cognitive function and brain health Helps manage blood sugar levels Reduces muscle pain and fatigue Supports overall immune system health Alpha Lipoic Blood sugar control, Nerve health, Neutralizes toxins $40 Read Benefits Acts as a powerful antioxidant, protecting cells from oxidative damage

These injections are typically less painful than intramuscular injections, which go into the muscle (requiring longer needles) (Usach, 2019)