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ajmalicine and tetrahydroalstonine showed higher binding affinities on alpha-2A, 2B, and 2C receptors, with K i values in the submicromolar range (K i = 1865 nM) than tetracyclic kratom alkaloids (K i values in the micromolar range)

In contrast, in mTYR, the Val377 and Ser380 are replaced with proline (P257) and alanine (A260), respectively, therefore it can be considered that there is little VdW clashes of the isopropyl group of -thujaplicin with A260 and P257 (Figure 16)