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D., Murray, I
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Discard the supernatant and wash three times with 1 ml of 3% (w/v) TCA resuspending each time the pellet with a glass rod
Our results provide a possible mechanism in which upregulation of phosphorylated p53 and p16 maintain the cell cycle arrest of keloid fibroblasts, and FOXO4-DRI selectively induces apoptosis of keloid senescent fibroblasts by prompting p53-pS15 nuclear exclusion
