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A topical anesthetic may be applied depending on the specific treatment

J Nat Prod 70:17461752 Carr G, Tay W, Bottriell H, Andersen SK, Mauk AG, Andersen RJ (2009) Plectosphaeroic acids A, B, and C, indoleamine 2,3-dioxygenase inhibitors produced in culture by a marine isolate of the fungus Plectosphaerella cucumerina

Guarducci C, Nardone A, Russo D, Nagy Z, Heraud C, Grinshpun A, et al

After the surgery was completed for each rat, atipamezole (1.5 mg/kg) was injected intraperitoneally
