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Apart from its role in DNA methylation, folate exhibits antioxidant properties that have been found to regulate the proliferation of cancer cells through three main mechanisms: (i) enhances the coupling of eNOS to cofactor tetrahydrobiopterin (BH4), hence inhibiting the formation of superoxide anion and the resulting peroxynitrite, a potent oxidant involved in various pathological conditions

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The supplementation also appeared to have a direct effect on immune function

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