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Finally, B12 is required to make myelin, the protective layer that coats nerve cells. B12 also works alongside folate to produce red blood cells, and without adequate B12 those cells grow abnormally large and lose their ability to carry oxygen efficiently

In the Billon 2023 paper, mice given 30 mg/kg IP still showed measurable plasma and muscle concentrations at 6 hours post-injection, which helps explain why twice-daily dosing was used in efficacy studies

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