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Description
To measure extracellular ROS production, the supernatant was treated with an Amplex Red assay reagent (Invitrogen, Carlsbad, CA, USA) and incubated at room temperature for 30 min
Future clinical trials may aim for higher methodological quality by integrating the following elements
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As a research-grade reagent, GHK-Cu serves as a reference standard and experimental tool across disciplines including biochemistry, cell biology, and materials science
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