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Description
After the ethanol was removed the next day, the cells were re-suspended in ice-cold PBS, treated with RNAse A (Keygen Biotech, China) at 37 C for 30 min, and then incubated with the DNA staining solution propidium iodide (PI, Keygen Biotech, China) at 4 C for 30 min
The trial used a flexible dosing protocol, permitting investigators to adjust doses based on individual tolerability and clinical judgement
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