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the transformed colonies were screened by colony PCR using T7 primers for the positive clones

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[Google Scholar] 8.Schauer P.R., Bhatt D.L., Kirwan J.P., Wolski K., Aminian A., Brethauer S.A., Navaneethan S.D., Singh R.P., Pothier C.E., Nissen S.E., Kashyap S.R., STAMPEDE Investigators Bariatric surgery versus intensive medical therapy for diabetes - 5-year outcomes
