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On the other hand, the first method HPLC method was stability indicating method and the paper applied very harsh conditions with insignificant degradation results, and the percentage degradation was ranging from 0.73 to 8.73% [14] which does not comply with ICH guidelines [15] and the other developed HPLC method for TIR quantitation employed gradient mobile phase elution and a relatively long separation time (30 min) [16]
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