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Genomic DNA (1 g) was converted using the ZYMO EZ DNA Methylation-Gold Kit (Zymo Research, lrvine, CA, USA) and one twentieth of the elution products were used as templates for PCR amplification with 35 cycles using KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems, Wilmington, MA, USA)

Cost of GLP-1 Agonists Therapy GLP-1 agonists, while highly effective for both type 2 diabetes and weight management, are associated with substantial healthcare costs, posing a significant economic burden on both individuals and healthcare systems