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Ensure the pen does not directly contact ice packs to prevent freezing, and track any time spent at room temperature toward the 21-day limit

To assess tirzepatide's impact on relapse-like behaviour, we utilised the alcohol deprivation effect (ADE) model 25 , 47 in a separate batch of male and female rats (n = 40, 10 per group) This protocol involved an 8-week baseline alcohol consumption period followed by 10-day alcohol deprivation within the intermittent access paradigm

Additionally, they inhibit the function of macrophages in the blood vessels and production of pro-inflammatory cytokines such as IL-6, IL-1, TNF-, and C-reactive protein (CRP)

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