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After the staining, the slices were washed by the PBS for at least 15 min/three times to washout secondary antibody

A 2023 study of more than 17,000 patients published in the New England Journal of Medicine found that semaglutide reduced the risk of major cardiovascular events including heart attack, stroke and cardiovascular death by about 20% in overweight or obese adults who already have cardiovascular disease but not diabetes

The latest diabetic retinopathy preferred practice guidelines from the American Academy of Ophthalmology indicate that the rising use of GLP-1RAs may lead to quick glycemic control and severe hypoglycemia and has been reported to cause higher rates of retinopathy complications, especially in patients with early-stage DR (Lim et al., 2025)