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Cultures were grown at 37 C for 68 h and subsequently shifted to 25 C for overnight protein expression

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GLP-1 is an energy sensor that reduces intestinal motility, prolonging the retention of food in the gut, contributing to more efficient absorption of nutrients and better bioavailability (Winkler et al., 2019)
Oxidative stress also seems to be an important pathophysiological mechanism of NAFLD (190)
