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This process is repeated until all amino acids that comprise GLP-1 are joined together, following which the peptide is purified through high-performance liquid chromatography

CHO cells expressing human GLP-1R were dispensed at 1 10 5 cells/mL in assay buffer (Hanks Balanced Salt Solution containing 0.1% BSA (Sigma-Aldrich) and 0.5 mM IBMX (Sigma-Aldrich)) in 384-well assay plates (5 L per well) before adding dilutions of tested compounds prepared by a manual tip-based method

Protein-to-Calorie Ratio: As previously detailed, this ratio is crucial for maximizing nutrient density

While they both promote weight loss, individual responses may vary