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Peptide cleavage from the resin solid support was performed using a 10 mL cleavage cocktail of TFA/H 2 O/TIPS (90:5:5, v/v/v), prepared before use in automation, with the individual components directly added to the SPPS reactor and mixed with low-pressure nitrogen flow for 2 h
Press the injection button or push the plunger slowly and steadily to deliver the full dose
