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D4H*-mCherry protein was eluted in elution buffer (25 mM L-glutathione, 50 mM Tris-HCl pH 8.8, 200 mM NaCl), filtered through a 30 kDa centrifugal filter unit (UFC203024, Thermo Fisher Scientific) and stored at -80C
Mol Cell Biochem (1982) 44:4964
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