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Description
Transition from metabolic healthy to unhealthy phenotypes and association with cardiovascular disease risk across BMI categories in 90 257 women (the Nurses Health Study): 30 year follow-up from a prospective cohort study
Cells were harvested by centrifugation at 4,000 g and resuspended in lysis buffer (20 mM Tris, 300 mM NaCl, 5 mM imidazole, 0.7 % CHAPS, pH 8.0) supplemented with protease inhibitors (Thermo Fisher Scientific) and bovine pancreatic DNase I (Sigma-Aldrich)
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doi: 10.1016/j.molmet.2018.12.001 [DOI] [PMC free article] [PubMed] [Google Scholar] 42.Adriaenssens AE, Biggs EK, Darwish T, et al
