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Serum biochemical analysis Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and fasting blood glucose (FBG) were quantified using an automatic biochemical analyzer (Hitachi, Tokyo, Japan) and commercial kits (FUJIFILM Wako Co., Ltd., Tokyo, Japan)

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