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Cells were then washed with KRBH and imaged in 3 mM glucose KRBH with images taken every 12 seconds in a 60/1.4 NA oil immersion objective with a 561 nm laser in a Nikon Eclipse Ti microscope with an ORCA-Flash 4.0 camera (Hamamatsu) and Metamorph software (Molecular Devices)

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This table shows the unadjusted analysis and adjusted analysis based on a Cox proportional hazards model with adjustment for baseline predictors of cardiorenal disease

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