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An analgesiometer Ugo Basile, Italy, Model 7200 that produces a mechanical force gradually increasing on the mid-gastrocnemius muscle of that animal's hind limb was utilized to detect the threshold of hind limb withdrawal
However, Sheehan et al
Reused needles become dull after a single use, which increases pain, tissue damage, and infection risk
refer to Appendix 4 for detailed summary)
