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In a similar protocol, the in vitro and in vivo effects of metformin on mitochondria function were investigated in vitro in primary murine hepatocytes and Hepa 1-6 cells (murine hepatoma cells derived from the BW7756 tumor) and an in vivo protocol with high-fat diet (HFD) treated mice (138)

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In this study, 788 patients completed the first 54 weeks and 517 patients continued through the 50-week extension study to complete a total of 104 weeks