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2.2 CCK-8 cell viability assay Logarithmically growing H9C2 cells were trypsinized, adjusted to a density of 5 10 3 cells/well, and seeded in 96-well plates with 100 L of medium per well
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In one study, women taking licorice root daily saw a significant drop in total testosterone levels within a month, though levels returned to normal after stopping the supplement
