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The serum insulin and glucagon levels were determined using a commercial Rat/Mouse Insulin enzyme-linked immunosorbent assay (ELISA) kit Merck Millipore (Kenilworth, NJ, USA) and by competitive inhibition enzyme immunoassay technique for quantitative measurement, using the enzyme-linked immunosorbent assay kit from Cloud-Clone Corp

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( 10.2174/1573399811309020007) [DOI] [PMC free article] [PubMed] [Google Scholar] 10.Nauck MA, Kemmeries G, Holst JJ, Meier JJ

In a recent observational study, individuals who discontinued semaglutide or tirzepatide within the first three months lost only 3.6% of their body weight, while those who quit between three and twelve months lost 6.8%