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"As I mentioned before, I kind of get that feeling of nausea, a weird taste in my mouth, and I find that water is really helpful." Users described water as a new craving, saying their mouth would start to feel dry around lunchtime if they hadnt finished enough early in the day

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Rosenstock J, Diamant M, Aroda VR, et al

After that, cells expressing GIPR and GLP-1R were incubated with 1:300 anti-Flag primary antibody (diluted with PBS containing 5% BSA, Sigma), and those expressing GCGR were incubated with 1:50 anti-GCGR antibody (diluted with PBS containing 5% BSA, Abcam) at RT for 1 h