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[37] More recently, Sakamoto and various co-authors [38] [39] have used random amplification of polymorphic DNA (RAPD) to isolate several genetic marker sequences that they name Male-Associated DNA in Cannabis (MADC), and which they interpret as indirect evidence of a male chromosome

Using a glass transfer pipette, wash down the sides of the tube five times with the sample, and transfer the sample to an autosampler vial with a screw cap and Teflon faced septa