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These include how often and how much tobacco they consume, biochemical markers (such as levels of cotinine, a by-product of nicotine metabolism) (covered in 12.5.6 Biomarkers of tobacco smoke and damage), and self-reported smoking behaviour measured in questionnaires

Importantly, in the NCI-MD cohort, genes of the mitochondrial component of the urea cycle, namely CPS1 , NAGS , and OTC , were the most significantly differentially expressed genes of the arginine metabolism pathway in CR hi tumors, and the mitochondrial component of the urea cycle pathway was identified as significantly downregulated in CR hi lung and liver tumors (Figure 5, G and H)

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