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After three rinses in PBS, slices were wet-mounted using Prolong Gold Antifade Reagent (Invitrogen, P36930)

If variable distribution was skewed, Kruskal-Wallis was applied with Mann-Whitney U-test in the post-hoc analyses (Table 1)

Tobacco products also contain acetaldehyde, which increases the addictive effects of nicotine, evidence such as a 2007 paper by Talhout et al., from European Neuropsychopharmacology confirms

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