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If one smokes this blend directly out of the tin, evidence of the humectant can be found both in the ash, and often in the amount of dottle remaining

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If plasmids did not have their own origin of replication, the replication process would be slower or even fail because the plasmids would have to compete for resources (as bacterial replication machinery is optimized for the replication of the bacterial chromosome, and it may not have enough resources like energy, necessary proteins or enzymes to efficiently replicate plasmids as well)

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