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The fusion expression vector was constructed using the pBI221 vector backbone, and restriction digestion with XbaI and SalI was performed on both the vector backbone and the PCR product
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Tobacco company PAC contributions to federal candidates, 2018 election cycle: More than $600,000 Annual tobacco industry expenditures lobbying Congress in 2016: $20.1 million Sources: Toll Of Tobacco In The United States Fact Sheet
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