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These results were fully reproduced by application of an acute pulse of nicotine in semi-intact preparation containing only the isolated heart with minimal nervous innervation (Dulcis and Levine, 2005

Collection of organoids To harvest organoids, pre-cooled Cell Recovery Solution (354253, Corning) was added to the Matrigel culture, followed by incubation on ice for 50 min with gentle shaking every 10 min to ensure uniform dissolution of the matrix while preserving the morphological integrity of the organoids

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The battery: Most Disposable vapes have a small lithium-ion battery, which contains various metals like copper, aluminum, and lithium