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Intracranial self-stimulation (ICSS) electrode implantation Mice were anaesthetized with a gas mixture of oxygen (1 L/min) and 13% of isoflurane (Piramal Healthcare, UK), then placed into a stereotaxic frame (Kopf Instruments, CA, USA)

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We found some evidence that genetic liability to depression increases smoking ( = 0.091, 95% CI 0.0270.155, p = 0.005) but evidence was mixed for schizophrenia ( = 0.022, 95% CI 0.0050.038, p = 0.009) with very weak evidence for an effect on smoking initiation

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