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Description
The extracts were purified on SepPak 500 mg silica cartridges (Waters), concentrated in SpeedVac to a final volume of 200 L, and analyzed by GC-MS as described.26 Carbonyl compounds.The content of the 2 DNPH-filled impingers (see smoke collection procedure above) was combined and analyzed for 8 carbonyl compounds by HPLC-UV.27,28 Briefly, an aliquot of the DNPH solution was mixed with 1% Trizma base solution to quench the DNPH reaction
Don't forget to stub your cigarette once you're done with it
Traces of gilding
It is important to properly dispose of them to avoid littering
